Totarol™

Raw Totarol
Raw Totarol

Mende Biotech’s primary product is Totarol™, a potent antibacterial, antimicrobial, antioxidant and anti-inflammatory compound extracted from recycled totara wood.

Totarol™ has international organic certification. Totarol™ extraction is a patented system and Mende Biotech is the only commercial supplier of Totarol™ in the world.

Totarol™ has a well-established name and brand within cosmetics and pharmaceutical industries and has an established market with the L’Oreal group of companies. The biggest growing market is in oral care, going from just New Zealand, into Taiwan and now Australia.

Totarol™ is unique

Super Critical Totarol™ has a unique signature which cannot be reproduced by any other means than our patented manufacturing technology.

Totarol Composition Document

COMPONENT CAS No. %
Totarol 511-15-9 60 ~ 85
Totarol hemiacetal ## 0 ~ 15
7a-Hydroxy totarol 6811-52-5 0 ~ 5
7-Oxototarol 13476-32-9 0 ~ 5
5-Hydroxy totarol ## 0 ~ 5
6,7-Dehydro totarol 3625-02-3 0 ~ 5
Ferruginol 514-62-5 0 ~ 5

Pure Totarol does NOT have the same activity with no reported activity on gram negative activity for example. We can produce pure Totarol but will require completely new testing, safety data and international registrations because it is quite different. All international testing over the last 15 years has been done on Totarol™. We believe the added activity like Totarol hemiacetal has extra activity not isolated in pure Totarol, hence we get gram negative activity.

We have developed a quantitative RPLC method to analyse Totarol levels which was developed in conjunction with Crop and Food Research in Dunedin.

Science

Background to Totarol™

Totara-8,11,13-trien-13-ol; C20H30O
Totara-8,11,13-trien-13-ol; C20H30O

Totarol is an aromatic diterpenoid with chemical formula C20H30O. Totarol is a natural extract from heartwood of the Totara tree (Podocarpus totara). Totara is a podocarp tree, unique to New Zealand. It has an ancient lineage up to 100 million years ago. Totara develops a massive trunk and can live to a great age. Totara can be found throughout New Zealand; in former times it was particularly abundant in the forests of the central North Island. Maoris revered Totara as a symbol of strength and used the wood for canoe-making because of its length, lightness and durability. The timber of Podocarpus totara is renowned for its resilience against rotting, which made it valuable to early European settlers of New Zealand for uses such as wharf piles, fence posts and foundation blocks. The durability stems from the anti-bacterial activity of Totarol™. Totarol™ can be extracted from the dead wood, negating the need to cut down live trees. It is also extractible from other podocarps including rimu; some trees in the cypress family (cypress, juniper, thuja); and rosemary. It is most abundant in Totara trees. The extraction process is called ‘supercritical extraction’. This is a process that uses high pressure carbon dioxide under defined conditions of temperature, pressure and gas flow to extract Totarol™ from powdered Totara deadwood (in the form of old fence posts, telegraph poles, house piles, and so on). The process ensures that the extract and residual wood has no harmful solvent residues, and that the highest possible quality of extract is obtained. This extraction process results in a product containing approximately 60-85% Totarol by mass.

Totarol™ is a broad-spectrum antibacterial, being active against Staphylococcus aureus; methicillin-resistant Staphylococcus aureus (epidemic, community, and multi-drug-resistant strains); Streptococcus mutans; penicillin-resistant Streptococcus pneumoniae; Erythromycin-resistant Streptococcus pyogenes; high-level-gentamicin-resistant Enterococcus faecalis; vancomycin-resistant Enterococcus faecalis; Salmonella menston Eschericia coli Enterobacter aerogenes Pseudomonas aeruginosa Bacillus subtilis Brevibacterium ammoniagenes; and Propionibacterium acnes Beta-amino alcohol derivatives of Totarol™ have demonstrated antiplasmodial properties that have no cross-resistance with chloroquine. Totarol™ has demonstrated activity against Mycobacterium tuberculosis.

Totarol™’s mechanism of antibacterial activity is not known, however some authors have suggested that it compromises the functional integrity of cell membranes. Haraguchi et al studied Totarol™’s actions on Pseudomonas and discovered that it inhibited oxygen consumption and respiratory-driven proton translocation in whole cells, and oxidation of NADH in membrane preparation (by inhibiting a number of NADH-related enzymes). Evans et al alternatively suggested that it disrupts bacterial energy metabolism, although they admitted that this action occurs at much higher concentrations than are significant for antibacterial activity. Although the exact mechanism of action is not known, all proposed mechanisms are dissimilar to those of macrolides and tetracyclines. Thus cross-resistance of macrolide- and tetracycline-resistant bacteria to Totarol™ seems unlikely.

Totarol™ is a potent anti-oxidant and strongly inhibits lipid peroxidation and scavenges ROS (reactive oxygen species) Totarol has a good antioxidant preservative activity in lipid or emulsion based systems which does not degenerate and why it combines well with GFX in the TGM dressing application.

Totarol™ is whitening

Dr Maeda from Japan has been working with Totarol™ with the features of his research being: The meleanin inhibitory effect in melanocytes is used for discovery of a whitening agent as a first screening. The growth-promoting effect in keratinocytes is used for a hair restorer. Moreover, the inhibitory effect of the collagen hardening by UVA irradiation is used for evaluation of an anti-photo ageing agent.

 

Totarol™ Processing